This guide will help you learn about reporter assays and find the one best suited for your needs. Visit the Promega catalog listing to view ordering information for the Reporter Assays featured here.
When creating a luciferase reporter assay, there are two important elements to consider: the luciferase reporter protein itself and the assay chemistry used to detect reporter activity. The characteristics of these two components together contribute to the overall performance of the assay. By selecting the reporter/detection solution that is optimal for your experimental goals, you can customize your luciferase reporter assay to create the best solution for your research.
Promega offers a choice of 3 different luciferase reporters: NanoLuc® Luciferase(Nluc,19kDa), Renilla Luciferase (Rluc, 36kDa) and Firefly Luciferase (Fluc, 61kDa) which vary in size, brightness, and protein half-life.
luc, luc+, luc2, luc2P, luc2CPLuc/Luc+ : Original luciferase gene used in older luciferase reporter systems.
Luc2 : Codon-optimized version of luciferase, engineered to remove most cryptic transcription factor binding sites and improve mammalian expression.
Luc2P and Luc2CP : Destabilized versions of Luc2 containing the PEST protein degradation sequence for rapid response.
Rluc, hRluc, hRlucP, hRlucCPRluc : Renilla luciferase.
hRluc : Synthetic codon-optimized Renilla luciferase, engineered to remove cryptic transcription factor binding sites for improved mammalian expression.
hRlucP and hRlucCP : Destabilized version of hRluc containing the PEST protein degradation sequence for rapid response.
Nluc, NlucP, secNlucNluc : NanoLuc luciferase.
NlucP : NanoLuc luciferase with PEST protein degradation sequence for rapid response.
secNluc : Secreted NanoLuc luciferase.
Multiple assay detection reagents are also available for each reporter. Key considerations for selecting the optimal assay reagent include: